TY  - JOUR
T1  - Comparison of microRNA Transcriptomes Between Immature and Mature Porcine Testes
AU - Li, Xuewei AU - Liu, Yihui AU - Ma, Jideng AU - Chen, Li AU - Lou, Pengbo AU - Zhou, Jun AU - Li, Mingzhou 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 3
SP  - 132
EP  - 138
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.132.138
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.132.138
KW  - High-throughput sequencing
KW  -microRNAs
KW  -transcriptome
KW  -porcine
KW  -testis
KW  -spermatogenesis
AB  - High-throughput sequencing of two small RNA libraries derived 
  from immature (20 days old) and mature (210 day old) porcine testis samples 
  yielded over 20 million high-quality reads. Researchers detected 461 mature 
  microRNAs (miRNAs) encoded by 277 precursor (pre)-miRNAs of which 428 were unique. 
  In total, 303 unique miRNAs of (428, 70.79%) were differentially expressed between 
  immature and mature porcine testes. Compared with immature testis, 95 unique 
  miRNAs were up-regulated and 208 unique miRNAs were down-regulated in mature 
  testis. Strikingly, researchers found that most miRNAs and differentially expressed 
  miRNAs were preferentially located on the X chromosome which implied their crucial 
  roles in the sex-determination system. Furthermore, GO and KEGG analyses of 
  the target genes that were predicted from the highly abundant differentially 
  expressed miRNAs between mature and immature porcine testes illustrate the likely 
  roles for these miRNAs in spermatogenesis. The study indicates that miRNAs are 
  extensively involved in spermatogenesis and that unraveling miRNA functions 
  in the testis will further the understanding of regulatory mechanisms of mammalian 
  spermatogenesis and male infertility treatment.
ER  -