TY  - JOUR
T1  - <I>In vitro</I> Optimization of White Yak (<I>Bos grunniens</I>) Oviduct Epithelial Cells Culture
AU - Yan, Ping AU - Guo, Xian AU - Ding, Xuezhi AU - Bao, Pengjia AU - Wang, Hongbo AU - Liang, Chunnian 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 1
SP  - 99
EP  - 103
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.99.103
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.99.103
KW  - White yak
KW  -oviduct epithelial cell
KW  -embryo
KW  -culture in vitro
KW  -China
AB  - Epithelial cells of the mammalian oviduct play an important 
  role in reproductive and developmental events. Therefore, the objectives of 
  the present study was to establish a method for isolation and culture of White 
  yak (<I>Bos grunniens</I>) oviduct epithelial cells <I>in vitro</I> and to determine 
  the effect of Fetal Calf Serum (FCS), Epidermal Growth Factor (EGF) and Transforming 
  Growth Factor-&#946;<SUB>1</SUB> (TGF-&#946;<SUB>1</SUB>) and their combination 
  on White yak oviduct epithelial cells. The results showed and 100 ng mL<SUP>-1</SUP> 
  of EGF in TCM 199 with 10% FCS could significantly increased cell numbers of 
  yak oviduct epithelial. Alone no addition, EGF (10 ng mL<SUP>-1</SUP>) and TGF-&#946;<SUB>1</SUB> 
  (1 ng mL<SUP>-1</SUP>) had no effect on cell number. However, when included 
  with 10% FCS in medium yak oviduct epithelial cell numbers significantly increased 
  on day 6 of culture. The combination of FCS (10%) and EGF (10 ng mL<SUP>-1</SUP>) 
  is the ideal formula to culture yak oviduct epithelial cells. Co-culture with 
  yak oviduct epithelial cells the developmental rate of blastocyst was 8.9%. 
  The results support the culture environment that oviduct epithelial cell may 
  have direct effects on yak embryo developmental block <I>in vitro</I> production.
ER  -