TY  - JOUR
T1  - Effects of Introns on the Regulation of Porcine Growth Hormone Expression
AU - Ju, Huiming AU - Bai, Lijing AU - Yang, Yuefei AU - Jiang, Xing AU - Sheng, Anzhi AU - Li, Kui 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 8
SP  - 892
EP  - 896
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.892.896
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.892.896
KW  - Intron
KW  -porcine growth hormone
KW  -pGH
KW  -gene expression
KW  -plasmids
AB  - It has been widely acknowledged that introns can influence 
  the efficiency of gene expression. Most transcriptional regulatory elements 
  are located within non-coding DNA. The aim of the study was to study introns 
  affecting porcine Growth Hormone (pGH) expression levels. A program was developed 
  for mammalian cells (PK15 cells) by measuring the enhancement effect on GH expression 
  of different introns inserted with otherwise identical plasmids. After selection 
  with G418, quantitative Polymerase Chain Reaction (qRT-PCR) assays was performed 
  to determine pGH mRNA expression levels. Western blotting was used to analyze 
  pGH protein expression. The qPCR and Western blot results revealed that various 
  introns have different effects on pGH expression efficiency. The recombinant 
  <I>pGH</I> gene with introns 3 and 4 had the highest expression activity. In 
  this study, researchers have provided the first insights into the function of 
  pGH introns, establishing a foundation for the recognition and better utilization 
  of these introns.
ER  -