TY  - JOUR
T1  - Molecular Phylogenetic Analysis of Non-Invasive Samples for the Endangered Phayre’s Leaf Monkey 
  (<I>Trachypithecus phayrei</I>) in Popa Mountain Park, Central Myanmar
AU - Lee, Woo-Shin AU - Thant, Naw May Lay AU - , Baek-Jun Kim AU - , Hyeon-Seo Ko AU - , Khin Mar Yi 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 5
SP  - 625
EP  - 632
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.625.632
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.625.632
KW  - Cytochrome b
KW  -non-invasive sample
KW  -phylogeny
KW  -protamine P1
KW  -Trachypithecus phayrei
KW  -Myanmar
AB  - Phayre&#146;s leaf 
  monkey (<I>Trachypithecus phayrei</I>) is an endangered endemic species distributed 
  predominantly in Southeast Asia. Although, three subspecies (<I>T. p. phayrei</I>, 
  <I>T. p. crepusculus</I> and <I>T. p. shanicus</I>) are known, molecular phylogenetic 
  studies on this monkey are still limited. In Myanmar, there was a controversy 
  for the species identity of Phayre&#146;s 
  leaf monkey inhabiting the Popa Mountain Park (PMP). Here, 32 non-invasive fecal 
  samples and one non-destructive bone sample were used to infer the phylogenetic 
  status of <I>T. phayrei</I> from PMP. Two DNA markers, nuclear Protamine P1 
  (Prm1) and mitochondrial Cytochrome b (Cyt-b) were selected for PCR and sequencing. 
  Three haplotypes for <I>Cyt-b</I> gene and two unique sequences for <I>Prm1</I> 
  gene were detected from 33 samples. The <I>Cyt-b</I> phylogenetic trees showed 
  that the population of Phayre&#146;s 
  leaf monkey in PMP is more closely related to the subspecies <I>T. p. shanicus</I>. 
  However, the Prm1 phylogenetic trees could not resolve the phylogenetic position 
  of <I>T. phayrei</I> subspecies. The results suggest the population from PMP 
  as the subspecies <I>T. p. shanicus</I> but further taxonomic studies for all 
  populations of this threatened monkey in Myanmar should be recommended for the 
  species&#146; conservation and management.
ER  -