TY  - JOUR
T1  - Characterization of HcMSP, A Novel Gender Specific Gene from Parasitic Nematode<I> Haemonchus contortus</I>
AU - Yan, Ruofeng AU - Zhang, Zhikai AU - , Lixin Xu AU - , Xiaokai Song AU - , Xiangrui Li 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 5
SP  - 593
EP  - 599
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.593.599
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.593.599
KW  - Haemonchus contortus
KW  -major sperm protein
KW  -RACE
KW  -differential expressions
KW  -gene
AB  - Major Sperm Protein (MSP) is one of the important components 
  of nematode sperms and the characters of this protein were widely studied in 
  <I>Caenorhabdit elegans</I> and <I>Ascaris suum</I>. However, no knowledge of 
  homology proteins in <I>Haemonchus contortus</I> was reported. In the present 
  research, the <I>MSP </I>gene of this parasite (HcMSP) was first cloned and 
  characterized. A pair of primer targeted the conserved sequence of nematode 
  MSPs was designed and a partial of <I>HcMSP</I> gene was obtained by reverse 
  transcription PCR (RT-PCR). Then, the 5' and 3' ends of <I>HcMSP</I> gene were 
  cloned by 5' Rapid Amplification of cDNA ends (RACE) and 3' RACE. The full length 
  of cDNA was obtained by overlapping the sequences of 3' and 5' extremities. 
  The Open Reading Frame (ORF) was amplified by RT-PCR and expressed in prokaryotic 
  cell.
ER  -