TY  - JOUR
T1  - Phylogenetic Identification and Distribution of Enterotoxin Genes in Aeromonas Strains Isolated from Pet Fish
AU - Shin, Gee-Wook AU - Kim, Dae-Cheol AU - , Seung-Won Yi AU - , Ho-Seong Cho AU - , Myung-Jo You 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 5
SP  - 557
EP  - 564
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.557.564
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.557.564
KW  - Aeromonas sp.
KW  -phylogenetic identification
KW  -gyrB
KW  -rpoD
KW  -enterotoxin gene
KW  -pet fish
AB  - In the present study, 47 strains of <I>Aeromonas</I> sp. were 
  collected from cultured koi (n = 11) and imported pet fish (n = 36). All strains 
  were identified by phylogenetic analysis using partial sequences of the <I>gyrB</I> 
  gene. In addition, PCR assays were used to detect the presence of genes for 
  cytotoxic enterotoxin (act) and cytotonic enterotoxin (alt and ast). In the 
  phylogenetic identification, the strains comprised five species, <I>A. veronii</I> 
  (n = 36), <I>A. hydrophila</I> (n = 7), <I>A. jandaei</I> (n = 1), <I>A. aquariorum</I> 
  (n = 1) and <I>A. allosaccarophila</I> (n = 2). Of the identified species, <I> 
  A. jandaei</I>, <I> A. aquariorum</I> and <I> A. allosaccarophila</I> were earlier 
  unrecorded in microbiological fields of Korea. In the detection of enterotoxin 
  genes, the <I>act</I>, <I>alt</I> and <I>ast</I> genes were respectively presented 
  in 85.1, 61.7 and 17.0% of strains. The act and <I>act/alt</I> gene patterns 
  were prominent among Aeromonas strains, especially <I>A. veronii</I>. On the 
  other hand, only <I>A. hydrophila</I> strains harbored all three of the <I>enterotoxin</I> 
  genes. The results of the present study suggest that pet fish could be a potential 
  risk factor for Aeromonas infection in humans.
ER  -