TY  - JOUR
T1  - ERK MAP-Kinase is Involved in the B7-H1 Expression Induced by Etoposide In Retinoblastoma Cells
AU - Chen, Zhen AU - Li, Junping AU - Yang, Anhuai AU - Huang, Linying AU - Liu, Zhigang AU - Zhou, Haixiao 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 3
SP  - 369
EP  - 376
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.369.376
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.369.376
KW  - mitogen-activated protein kinases
KW  -etoposide
KW  -B7-H1
KW  -Retinoblastoma
KW  -small interfering RNA
AB  - The effect of etoposide on B7-H1 expression was determined by the Reverse-Transcription Polymerase Chain Re-action (RT-PCR), real-time PCR and flow cytometry analysis in Y79 cells. Then, the involvement of Mitogen-Activated Protein Kinases (MAPKs) signal pathways were tested by Western blotting and signal transduction inhibitor assays. Furthmore, specific small interfering RNA (siRNA) targeting B7-H1 was transfected into Y79 retinoblastoma cells using liposome. Silencing of B7-H1 expression was measured by RT-PCR and Western blotting assays. Etoposide increased B7-H1 mRNA and protein levels in Y79 cells. The effect of etoposide on B7-H1 expression peaked at the concentration of 5 &#956;g mL<SUP>-1</SUP> as confirmed by RT-PCR, real-time PCR and flow cytometry assays (p&lt;0.01). The phosphorylation status of Extracellular Signal-Regulated Kinase (ERK), c-Jun N-terminal Kinase (JNK) were constitutively activated by etoposide and MEK inhibitor simultaneously reduced the expression of B7-H1 induced by etoposide (p&lt;0.01). B7-H1 siRNA significantly silenced B7-H1 expression in Y79 cells as confirmed by RT-PCR and Western blotting assays (p&lt;0.01).
ER  -