TY  - JOUR
T1  - A Species Boundary Within the <I>Tylototriton verrucosus</I> Group (Urodela: Salamandroidae) Based on Mitochondrial DNA Sequence Evidence
AU - Li, Yan AU - Zhang, Mingwang AU - Yu, Guohua AU - Rao, Dingqi AU - Huang, Yimei AU - Yang, Junxing 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 3
SP  - 337
EP  - 343
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.337.343
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.337.343
KW  - Species boundary
KW  -Tylototriton verrucosus group
KW  -red knobby newt (Tylototriton shanjing)
KW  -cyt b gene
KW  -clades
AB  - The taxonomic status of red knobby newt (<I>Tylototriton shanjing</I>) is under dispute. Molecular phylogenetic tree of <I>Tylototriton verrucosus</I> group was reconstructed based on 753 bp of partial mitochondrial <I>cyt b</I> gene sequence to determine species boundaries among the species in <I>T. verrucosus</I> group. The phylogeny result indicates that four major clades (clade I-IV) can be distinguished within <I>Tylototriton verrucosus</I> group. Clade I and IV consist of <I>T. taliangensis</I> and <I>T. kweichowensis</I>, respectively. Clade II consists of samples of <I>T. shanjing</I> derived from Yunnan of China, a form that researchers resurrect from its synonym under <I>T. verrucosus</I>. <I>T. verrucosus</I> haplotypes from Shan State of Myanmar and pet trade formed clade III. All populations of <I>T. shanjing</I> and <I>T. verrucosus</I> formed strongly supported (PP = 1.0) reciprocal monophyletic groups. The average uncorrected pairwise genetic distance (p-distance) of cyt b between these four clades ranges from 0.060-0.089 which is obviously higher than within these four major clades (0.001-0.014). Researchers propose that the <I>T. shanjing</I> should be a valid species rather than synonym of <I>T. verrucosus</I>.
ER  -