TY  - JOUR
T1  - Identification of Polymorphisms in the Bovine Paraoxonase 1 Gene
AU - Wu, Jianping AU - Zhang, Juan AU - Zhao, Shengguo AU - Lei, Zhaoming AU - Wang, Xinrong AU - A. Brown, Michael 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 2
SP  - 229
EP  - 233
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.229.233
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.229.233
KW  - Cattle
KW  -PON1 gene
KW  -exon
KW  -polymorphism
KW  -PCR-SSCP
KW  -China
AB  - The Paraoxonase 1 gene (<I>PON1</I>) encodes paraoxonase which 
  hydrolyzes organophosphate pesticides and nerve gasses. In addition, PON1 has 
  been shown to have significant antiatherosclerotic effects and is a major candidate 
  gene associated with the reduction of atherosclerosis. Most of the literature 
  reporting polymorphisms in PON1 has been in humans while less information about 
  the polymorphism of <I>PON1</I> gene has been reported in mammals, especially 
  in bovine species. Polymorphisms in the <I>PON1</I> gene were evaluated in Simmental 
  x Chinese Yellow cattle (n = 32) and Longdong cattle (n = 37) using PCR-SSCP 
  in exons 1-9 of the <I>PON1</I> gene. Results demonstrated polymorphisms in 
  the bovine <I>PON1</I> gene at exons 4, 6, 8 and 9. DNA sequencing identified 
  specific substitutions in exon 4, 6, 8 and 9 (g.338G&gt;A, g.647C&gt;T, g.896C&gt;T 
  and g.998T&gt;C, respectively). Two genotypes were identified in exon 4 (GG 
  and AG), exon 6 (TT and CT) and exon 9 (TT and CT) while 3 genotypes were identified 
  in exon 8 (CC, CT and TT). The allelic frequency for the G allele was 92.03% 
  and the A allele was 7.97% in exon 4. Allelic frequencies for the T and C alleles 
  were 89.86 and 10.14%, respectively, in exon 6. In exon 8, frequencies for the 
  C and T alleles were 94.93 and 5.07%, respectively. In exon 9, allelic frequencies 
  for the T and C alleles were 88.41 and 11.59%, respectively.
ER  -