TY  - JOUR
T1  - Study of MMP-2 and FAK Expression in HSV-1 Infected Human Corneal Epithelial Cells
AU - Yiqiao, Caoting Xing AU - Yanning, Yang AU - Xiaoxiong, Wang 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 22
SP  - 1632
EP  - 1637
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1632.1637
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1632.1637
KW  - MMP-2
KW  -FAK
KW  -HSV-1 keratitis
KW  -virus
KW  -cells
AB  - To investigate Matrix Metallo Proteinases-2 (MMP-2) and Focal Adhesion Kinase (FAK) expression in Herpes Simplex Virus-1 (HSV-1) infected human corneal epithelial cells. Human corneal epithelial cells were infected with HSV-1 <I>in vitro</I> to establish cell model of viral infection. The expression of MMP-2 and FAK were detected by RT-PCR, Western blot, Immunohistochemical and Immumofluorescence Method at 2, 20 and 40 h post-infection. Results at 2, 20 and 20 h post-infection, the mRNA of MMP-2 and FAK expression were significantly increased compared with uninfected cells (p&lt;0.01, p&lt;0.05). At 2 h post-infection, the expression of MMP-2, FAK and Phosphorylation FAK (P-FAK) protein were not significantly different with uninfected cells (p&gt;0.05). At 20 and 40 h post-infection, the expression of MMP-2, FAK and P-FAK protein were significantly increased compared with uninfected cells (p&lt;0.05). At the initial stage of HSV-1 infected, P-FAK plays an important role in the process of virus invading and MMP-2 activation.
ER  -