TY  - JOUR
T1  - Immunity Efficacy of a DNA Vaccine Encoding VirB8 of Brucella in BALB/c Mice
AU - Guizhen, Wang AU - Baoshan, Liu AU - Bo, Chen 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 2
SP  - 160
EP  - 167
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.160.167
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.160.167
KW  - Brucella
KW  -VirB8
KW  -DNA vaccine
KW  -immunity efficacy
KW  -in vitro
AB  - Brucella is a intracellular bacterial pathogen. Due to current 
  available attenuated vaccine against it has certain defects. The development 
  of an effective DNA vaccine against brucellosis is an area of intense research. 
  This study was conducted to evaluate the immunogenicity and protective efficacy 
  of a DNA vaccine encoding Brucella VirB8 which is a essential components of 
  type IV Secretion Systems (T4SSs) and bacterial virulence factor. The BALB/c 
  mice inoculated Intramuscularly with pCDNA-VirB8 plasmid developed both humoral 
  and cellular immune responses. They produced VirB8-specific antibodies with 
  a dominance of IgG2a over IgG1 and a T-cell-proliferative response. Recombinant 
  VirB8 also induced the production of gamma Interferon but not Interleukin-4 
  (IL-4) after stimulating lymphocytes with it <I>in vitro</I>. This suggested 
  the induction of a typical T-helper-1-dominated immune response in mice. The 
  protective ability of the DNA vaccine was evaluated by systemic bacterial clearance 
  after challenging the mice with S2. The pCDNA-VirB8 induced a significant level 
  of protection in BALB/c mice. Altogether, these data suggest that VirB8 is a 
  good candidate for studies of DNA vaccination against brucellosis in future.
ER  -