TY  - JOUR
T1  - Epigenetic Regulation of Methylated CpG Binding Protein MBD1 and its Effect on Developmental Gene Expression in the Bovine Embryo
AU - Yu, HaiQuan AU - Wang, HuiMin AU - Ao, XuDong AU - Wang, ZhenFei AU - Zhang, Na AU - Gao, HaiXia AU - Bai, HaiDong AU - Yue, YongLi 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 21
SP  - 1597
EP  - 1604
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1597.1604
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1597.1604
KW  - MBD1
KW  -early embryonic development
KW  -epigenetic regulation
KW  -pluripotency genes
KW  -maternal genes
AB  - To elucidate the epigenetic regulation of methylated CpG binding 
  protein (<I>MBD1</I>) gene expression and its effect on other developmental 
  genes, the expression of MBD1 and the DNA methylation status of its regulatory 
  region were investigated in differentiated bovine tissues, during oocyte maturation 
  and in the preimplantation embryo. Firstly, <I>MBD1</I> gene expression was 
  analyzed by RT-PCR in bovine heart, liver, kidney, testis and ovary and at different 
  stages of oocyte maturation and embryonic development. Dynamic changes in DNA 
  methylation in the regulatory region of MBD1 were detected by bisulfite-sequencing 
  and chromosome immunoprecipitation was used to investigate the relationship 
  between MBD1 and the pluripotency genes <I>Nanog</I>, <I>Oct4</I> and <I>H1foo</I> 
  or the maternal gene <I>ZAR1</I>. The results showed that the transcription 
  level of the <I>MBD1</I> gene was lower in heart and kidney and the DNA methylation 
  levels of the MBD1 regulatory region were also lower in heart and kidney than 
  in liver, testis and ovary. The transcription level of MBD1 incresed stably 
  during the <I>in vitro</I> maturation of oocytes but began to decline dramatically 
  from the 4 cells embryo stage. However, the DNA methylation levels of the MBD1 
  regulatory region were identical during either oocyte maturation or embryonic 
  development. MBD1 protein spatiotemporally binded to the promoter regions of 
  the pluripotency genes <I>Nanog</I> and<I> Oct4</I> and the maternal genes <I>ZAR1</I> 
  and <I>H1foo</I>. These results indicated that during the bovine preimplantation 
  embryo stages from the germinal vesicle oocyte to the blastocyst, transcription 
  of MBD1 was not controled by the mechanism of promoter DNA methylation and MBD1 
  protein may participates in the transcriptional regulation of some key developmental 
  regulatory genes.
ER  -