TY  - JOUR
T1  - Differentiation of Pancreatic Duct-Derived Stem Cells of Rats into Insulin-Secreting Cells with Function of Down-Regulating Blood Sugar Level after Transplantation
AU - Jin, Bo AU - Liu, Yan-Ping AU - Wang, Hai-Jiang 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 20
SP  - 1565
EP  - 1573
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1565.1573
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1565.1573
KW  - pancreatic duct-derived stem cell
KW  -diabetes
KW  -islet transplantation
KW  -mesenchymal stem cell
KW  -pancreatic stem cell
KW  -Stem cell
AB  - To induce Pancreatic Duct-derived Stem Cells (PDSCs) of rats 
  into insulin-secreting cells and to determine the <I>in vitro</I> and <I>in 
  vivo</I> function of the insulin-secreting cells. PDSCs were obtained through 
  <I>in situ</I> collagenase digestion of rat panrease and <I>in vitro</I> adherent 
  cultivation, with the phenotype identified and then PDSCs were induced into 
  Islet-like Cell Clusters (ICCs) through serum-free culture with their response 
  to glucose challenge determined. Furthermore, ICCs were transplanted under the 
  kidney capsule of diabetic nudes, with their blood sugar level monitored to 
  determine the <I>in vivo</I> function of ICCs on regulating blood sugar. PDSCs 
  of rats resembled Mesenchymal Stem Cells (MSCs) with CD34 and CD45 positive 
  and CD90 and CD105 negative on phenotype, simultaneously with the expression 
  of PDX-1 and nestin. PDSCs could differentiated into insulin-secreting ICCs 
  through serum-free cultivation. After transplantation under the kidney capsule, 
  ICCs could just softly down-regulated the blood sugar level of diabetic recepient 
  nudes, showing the immaturity of ICCs. PDSCs of rats resembled MSCs, with the 
  expression of PDX-1 and nestin which could differentiated into insulin-secreting 
  ICCs <I>in vitro</I> and down-regulated the blood sugar level of diabetic recepient 
  nudes after transplantation.
ER  -