TY  - JOUR
T1  - Optimizing the Method for Cleavage of Ssp DnaB Mini-Intein Mediated Precursor Proteins
AU - Yuan, Lixing AU - Tong, Yu AU - Gao, Ju AU - Zhou, Jing 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 20
SP  - 1537
EP  - 1540
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1537.1540
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1537.1540
KW  - Optimizing the Method for Cleavage of Ssp DnaB Mini-Intein Mediated Precursor Proteins
KW  -cleavage
KW  -C-extein
KW  -pH
KW  -protein
AB  - Inteins are protein splicing elements that excise themselves 
  from precursor protein. It has been widely used to purify recombinant proteins. 
  In the current study, using the Impact-Twin System to express the fusion protein 
  CBD-Ssp DnaB intein-C-extein, researchers investigated the first C-extein residue 
  that may influence the cleavage of Ssp DnaB intein. The results showed that 
  cleavage of the fusion precursor protein was temperature and time dependent 
  and was optimal when pH was 7.0-7.5. Efficiency of cleavage was also, dependent 
  on the first residue of C-extein as well as time and temperature. The data may 
  help in elaborating a facile method for producing other proteins of interest.
ER  -