TY  - JOUR
T1  - Establishment and Biological Characteristic Research of Altay Sheep Fibroblast 
  Cell Bank
AU - Zhao, Yuhua AU - Wang, Hui AU - He, Xiaohong AU - Lu, Taofeng AU - Zhu, Zhiqiang AU - Guan, Weijun AU - Ma, Yuehui 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 20
SP  - 1530
EP  - 1536
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1530.1536
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1530.1536
KW  - Altay sheep
KW  -fibroblast line
KW  -biological characterization
KW  -genetic resources conservation
KW  -somatic cells cryopreservation
AB  - A fibroblast bank of Altay sheep consisting of 182 tubes of 
  frozen cells was successfully abtained from primary explants of 57 individual 
  animals through attachment cultivation and cryopreservation biotechniques and 
  each vial contains 1.3x10<SUP>7</SUP> cells, respectively. The results of quality 
  assays and biological characteristic researches showed that the cells cultured 
  with a Population Doubling Time (PDT) of about 24 h, constituted a typical fibroblast 
  in morphology; assays for microbial contamination regarding bacteria, fungi, 
  viruses and mycoplasmas are all negative; analysis of Lactate Dehydrogenase 
  (LDH) and Malate Dehydrogenase (MDH) isoenzymes ruled out the possibilities 
  of cross-contamination between species; the ratio of diploid cells is 98.6%. 
  In order to study the expressibility of exogenous genes, six kinds of fluorescent 
  protein-coding plasmids were transfected into the Altay sheep fibroblast with 
  transfection efficiencies between 22.3 and 46.7%. This research could not only 
  preserve the genetic resources of Altay sheep but also provide both technical 
  and theoretical basis for preserving those of other mammals and poultry at cell 
  level.
ER  -