TY  - JOUR
T1  - A <I>hsp70</I> Gene-Based PCR for Detection of <I>Mycoplasma ovipneumoniae
AU - Yue, Hua AU - Tang, Cheng AU - Han, Xiao AU - Yang, Falong 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 19
SP  - 1495
EP  - 1497
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1495.1497
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1495.1497
KW  - hsp70 gene
KW  -polymerase chain reaction
KW  -Mycoplasma ovipneumoniae
KW  -detection
KW  -China
AB  - <I>Mycoplasma ovipneumoniae</I> is associated with atypical pneumonia in sheep 
  and goats. Traditional detection of <I>M. ovipneumoniae</I> can be complex owing 
  to its slow growth and fastidious nutritional requirements. In present study, 
  researchers developed a PCR Assay Method for specific and sensitive detection 
  of <I>M. ovipneumoniae</I> using primers targeting the heat shock protein 70 
  gene (<I>hsp70</I>). The results demonstrated that the <I>hsp70</I> gene-based 
  PCR is specific by producing an amplicon of 135 bp only with <I>M. ovipneumoniae</I> 
  strains. The detection limit is 4.4 pg which is 10 times more sensitive than 
  the existing 16S rDNA-based PCR. The <I>hsp</I>70 gene-based PCR also demonstrated 
  a higher detection rates for both nasal swabs and lung tissues when comparing 
  with 16S rDNA-based PCR.
ER  -