TY - JOUR
T1 - Development and Application of a Polymerase Chain Reaction to Early Detect
Haemophilus parasuis
AU - Xu, Zhiwen AU - Zhicai, Zuo AU - Dai, Hongbo AU - Chen, Lei AU - Zhou, Yuancheng AU - Guo, Wanzhu
JO - Journal of Animal and Veterinary Advances
VL - 12
IS - 2
SP - 140
EP - 145
PY - 2013
DA - 2001/08/19
SN - 1680-5593
DO - javaa.2013.140.145
UR - https://makhillpublications.co/view-article.php?doi=javaa.2013.140.145
KW - Haemophilus parasuis
KW -OMP
KW -PCR
KW -early detection
KW -blood
AB - Haemophilus parasuis mainly affect piglets during suckling
period and the infected pigs showed clinical symptoms at 5-6 weeks old after
weaning. To develop a specific PCR for early detection of Haemophilus parasuis,
early treatment would effectively prevent phenotypic expression of the disease.
A pair of specific primers were designed to amplify a 414 bp specific DNA fragment
based on H. parasuis Outer Membrane Protein (OMP) gene published
in GenBank also the PCR conditions were optimized. The established PCR test
could detect a minimum of 425 cfu mL-1 (0.425 cfu/PCR reaction) pure
cultures of H. parasuis and only H. parasuis could amplify a 414
bp specific DNA fragment. No amplification was observed while tested with the
closely affiliated species and the mainly contaminating flora. From 2 days post
infection (dpi) to 5 dpi, all of the piglets challenged with H. parasuis
showed a fever above 41°C and all blood samples from those feverish piglets
were positive for H. parasuis. Through blood PCR detection, 37 positive
samples were picked out in 84 sucking pigs which were natural infected with
H. parasuis, the result was verified by dissecting five positive piglets
and isolating bacteria from lung tissue. The developed PCR was successfully
applied for early diagnosis of H. parasuis infection, early treatment
will reduce the incidence and mortality of disease.
ER -