TY  - JOUR
T1  - Molecular Characterization and Expression Pattern of a Novel Flavonoid 3'-Hydroxylase 
  Gene
AU - Leifeng, Zhao AU - Fuzhao, Nian 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 16
SP  - 1352
EP  - 1356
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1352.1356
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1352.1356
KW  - Flavonoid
KW  -tobacco
KW  -flavonoid 3`-hydroxylase
KW  -expression pattern
KW  -flower
AB  - Flavonoids have significant health benefits for animals and humans. Flavonoid 3'-hydroxylase gene have been characterized to be required for flavonoid biosynthesis. In present experiment, the complete mRNA sequence of tobacco flavonoid 3'-hydroxylase gene was amplified using the rapid amplification of cDNA ends methods. The full-length tobacco flavonoid 3'-hydroxylase gene mRNA was 1828 bp containing an 1578 bp open reading frame which encodes a protein of 515 amino acids. Sequence analysis revealed that the flavonoid 3'-hydroxylase genes are conserved in plants. Results also showed that tobacco flavonoid 3'-hydroxylase gene has a closer genetic relationship with the flavonoid 3'-hydroxylase gene of <I>Lycopersicon esculentum</I>. Prediction of transmembrane helices showed that tobacco flavonoid 3'-hydroxylase might be a transmembrane protein. The expression profile was studied and the results indicated that tobacco flavonoid 3'-hydroxylase gene was highly expressed in in leaf and flower. These results established the primary foundation of utilization of tobacco flavonoids in the future.
ER  -