TY  - JOUR
T1  - Phylogenetic Relationships of the <I>18SrRNA </I>Gene Sequence of <I>Histomonas 
  meleagridis</I> from Chickens in East China
AU - Tao, Jianping AU - Xu, Jinjun AU - Qu, Chanbao AU - Guo, Ping AU - Liu, Chong 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 16
SP  - 1338
EP  - 1342
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1338.1342
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1338.1342
KW  - Histomonas meleagridis
KW  -18SrRNA
KW  -homology
KW  -phylogenetic relationships
KW  -China
AB  - <I>Histomonas meleagridis</I> (<I>H. meleagridis</I>) is a protozoan parasite that may cause histomoniasis, a disease in poultry of the order Galliformes. The phylogenetic relationships between <I>H. meleagridis</I> in different geographical regions of China are not well understood. By PCR, the partial <I>18SrRNA </I>gene was amplified from 30 liver samples collected from chickens infected with <I>H. meleagridis</I> in East China. The obtained sequences were aligned with other known <I>18SrRNA</I> gene sequences of <I>H. meleagridis </I>strains and two taxonomically related protozoan species. The alignment results showed that the <I>18SrRNA</I> gene sequence of <I>H. meleagridis</I> was highly conserved in East China with nucleotide sequence identity of 98.4-100.0%. Further phylogenetic analysis revealed these sequences were clustered into five groups, suggesting the presence of different genotypes. These results throw a light on molecular diagnosis, epidemiological investigation and population genetics of <I>H. meleagridis</I> in China.
ER  -