TY  - JOUR
T1  - Molecular Characterization and Expression Pattern of a Novel <I>NMNAT1</I> 
  Gene
AU - Leifeng, Zhao AU - Fuzhao, Nian AU - Yonghui, Zhang AU - Dingqi, Luo AU - Jianhua, Xia AU - Sheng, Jiang 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 15
SP  - 1281
EP  - 1285
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1281.1285
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1281.1285
KW  - Tobacco
KW  -gene
KW  -NMNAT1
KW  -expression pattern
KW  -dinucleotide
AB  - Nicotinamide adenine dinucleotide is important to the health 
  of animals and humans. Nicotinamide Mononucleotide Adenylyltransferase 1 (<I>NMNAT1</I>) 
  gene has been characterized to be required for nicotinamide adenine dinucleotide 
  biosynthesis. In present experiment, the complete mRNA sequence of tobacco <I>NMNAT1</I> 
  gene was amplified using the rapid amplification of cDNA ends methods. The full-length 
  tobacco <I>NMNAT1</I> gene mRNA was 1,445 bp containing a 753 bp open reading 
  frame which encodes a protein of 250 amino acids. BLAST analysis revealed that 
  tobacco NMNAT1 protein shares high homology with the NMNAT1 of <I>Lycopersicon 
  esculentum</I> (87%), wine grape (65%), soybean (65%), cacao (65%) and barrel 
  medic (62%). Results also showed that tobacco <I>NMNAT1</I> gene has a closer 
  genetic relationship with the <I>NMNAT1</I> gene of <I>lycopersicon esculentum</I>. 
  The expression profile was studied and the results indicated that tobacco <I>NMNAT1</I> 
  gene was highly expressed in leaf. These results established the primary foundation 
  of utilizing tobacco nicotinamide adenine dinucleotide or NMNAT1 as drugs for 
  animals and humans in the future.
ER  -