TY  - JOUR
T1  - Cloning and Analysis of Leptin in <I>Culter alburnus</I> in Xingkai Lake 
  and down Regulating its Expression Compared to Cultured Population
AU - Wang, Kun AU - Chen, XiaoTing AU - Zhao, RongWei AU - Hao, QiRui AU - Hao, QiRui AU - Xu, GeFeng AU - Jiang, XuYang AU - Han, Ying 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 1
SP  - 128
EP  - 134
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.128.134
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.128.134
KW  - Culter alburnus
KW  -Xingkai Lake
KW  -Leptin
KW  -cloning
KW  -expression
KW  -wild and cultured population
AB  - Researchers cloned the Leptin of <I>Culter alburnus</I> in 
  Xingkai Lake and analyzed the sequence characteristic of Leptin coding protein 
  and detected the relative expression levels of Leptin RNA in wild and cultured 
  <I>Culter alburnus</I> in Xingkai Lake of different ages. The result provide 
  reference on studying the regulatory effect and molecular mechanism of Leptin 
  on food intake, digestion and catabolism genes and in <I>Culter alburnus</I> 
  in Xingkai Lake. The full length cDNA of <I> Leptin</I> gene was got by RT-PCR, 
  5'-RACE and 3'-RACE. The primer of semi-quantitative RT-PCR and real-time PCR 
  were designed by the acquired cDNA then the gene expression was studied. The 
  full sequence length of Leptin in <I>Culter alburnus</I> in Xingkai Lake was 
  1208 bp, the ORF length is 522 bp which code 173 amino acids. With the increase 
  of age, the expression of mRNA in both wild and cultured <I>Culter alburnus</I> 
  in Xingkai Lake show rising trend, from 3 years old, the expression of Leptin 
  in wild population showed down regulation in contrast to the cultured population.
ER  -