TY  - JOUR
T1  - Molecular Characterization and Expression Pattern of a Novel Peroxisomal Nicotinamide Adenine Dinucleotide Carrier Gene
AU - Leifeng, Zhao AU - Sheng, Jiang AU - Jianhua, Xia AU - Dingqi, Luo AU - Yonghui, Zhang AU - Fuzhao, Nian 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 15
SP  - 1276
EP  - 1280
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1276.1280
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1276.1280
KW  - expression pattern
KW  -peroxisomal nicotinamide adenine dinucleotide carrier
KW  -gene
KW  -Tobacco
KW  -millet
AB  - Nicotinamide adenine dinucleotide have significant health 
  benefits for animals and humans. Peroxisomal nicotinamide adenine dinucleotide 
  carrier gene functions in mediating the nicotinamide adenine dinucleotide import 
  into peroxisomes. The complete coding sequence of tobacco peroxisomal nicotinamide 
  adenine dinucleotide carrier gene was amplified by RT-PCR. The open reading 
  frame of tobacco peroxisomal nicotinamide adenine dinucleotide carrier gene 
  was 1017 bp which encodes a protein of 338 amino acids. Sequence analysis revealed 
  that the peroxisomal nicotinamide adenine dinucleotide carrier of tobacco shares 
  high homology with the peroxisomal nicotinamide adenine dinucleotide carrier 
  of <I>Lycopersicon esculentum</I> (88%), <I>Fragaria vesca</I> subsp. vesca 
  (74%), thale cress (73%), chickpea (73%) and foxtail millet (67%). Phylogenetic 
  tree analysis revealed that the tobacco peroxisomal nicotinamide adenine dinucleotide 
  carrier gene has a closer genetic relationship with that of <I>Lycopersicon 
  esculentum</I>. Prediction of transmembrane helices showed that tobacco peroxisomal 
  nicotinamide adenine dinucleotide carrier might be a transmembrane protein. 
  Expression profile was studied and the results indicated that tobacco peroxisomal 
  nicotinamide adenine dinucleotide carrier gene was highly expressed in stem, 
  leaf and flower. These results established the primary foundation of using tobacco 
  nicotinamide adenine dinucleotide as drug for animals and humans in the future.
ER  -