TY  - JOUR
T1  - Study on Mechanism for P21/53 Promoting Invasion of Human Glioma Stem Cells by Inhibiting TIMP-2 Expression
AU - Xu, YingHui AU - Fu, Xin AU - Li, Weihua AU - Zhang, Jian 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 14
SP  - 1220
EP  - 1227
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1220.1227
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1220.1227
KW  - P21
KW  -P53
KW  -TIMP-2
KW  -human glioma stem cells
KW  -invasion
AB  - To study the role of P21/53 in directly regulating and controlling 
  TIMP-2 and the important mechanism for promoting invasion of GSCs by P21/53. 
  Transwell <I>in vitro</I> invasion experiment was used to test invasion ability 
  of GSCs and GCs as well as effect of P21/53 on GSCs invasion. Techniques of 
  qRT-PCR and Western blot were used to test and transfect expression variation 
  of TIMP-2, MMP2 and MMP9 in GSCs of P21/53 inhibitor. The luciferase reporter 
  gene experiment was used to test the role of P21/53 in directly regulating and 
  controlling TIMP-2. The number of invasive GSCs significantly decreased following 
  transfection of P21/53 inhibitor (p&lt;0.05). The expression levels of TIMP-2 
  mRNA in the U87 cell line and GSCs from the two primary glioma specimens rose 
  compared with the control group, respectively while the expression levels of 
  MMP2 mRNA and MMP9 mRNA fell compared with the control group (p&lt;0.05) following 
  transfection of P21 inhibitor. Expression of protein level of TIMP-2, MMP2 and 
  MMP9 were consistent with mRNA level and had significant difference compared 
  with those of the control group following transfection of P21 inhibitor and 
  P53 inhibitor. The luciferase activity of the reporter vector containing wiled-type 
  TIMP-2-3'UTR increased significantly following transfection of P21/53 while 
  the luciferase activity of the reporter decreased significantly following transfection 
  of P21/53 simulator (p&lt;0.05). High expression of P21/53 promotes invasiveness 
  of GSCs and its mechanism is achieved by down-regulating expressions of TIMP-2 
  and up-regulating expressions of MMP2 and MMP9 as a minimum.
ER  -