TY  - JOUR
T1  - Efficiency of Exogenous Gene Introduction into Chicken Primordial
 Germ Cells for Producing Transgenic Chicken
AU - Furuta, Hiroki AU - Tanaka, Mimaki AU - Fujiia, Yuuna AU - Yoshida, Tatsuyuki 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 14
SP  - 1198
EP  - 1201
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1198.1201
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1198.1201
KW  - Transgenic chicken
KW  -PGCs
KW  -GFP
KW  -lipofection
KW  -electroporation
AB  - It has been investigated that transgenic chicken can be produced 
  by various methods by introducing an exogenous gene into chicken Primordial 
  Germ Cells (PGCs). Blood containing PGCs was collected from the blood vessels 
  of embryos at stages 12-15. An exogenous gene encoding Green Fluorescent Protein 
  (GFP) was introduced into the PGCs by lipofection and electroporation. Electroporation 
  was carried out under 200 V/25 μF, 200 V/200 μF and 200 V/950 μF. 
  GFP expression was observed in PGCs. Efficiency of introduction into PGCs was 
  low. Electroporation was superior to lipofection for the introduction of the 
  exogenous gene into chicken PGCs. PGCs treated for GFP introduction by electroporation 
  were injected into blood vessels of recipient embryos at stages 12-15. After 
  incubation until stage 26, GFP bands were obtained from gonads of embryos, thereby 
  demonstrating that treated PGCs had migrated to the gonad.
ER  -