TY  - JOUR
T1  - Phylogenetic Analysis and Detection of Related Genes of High-Pathogenicity Island of <I>Escherichia coli</I> Strains Isolated from Chicken in Hebei Province, China
AU - Shi, Qiumei AU - Zhang, Yanying AU - Gao, Guisheng AU - Fang, Hai AU - Chen, Cuizhen AU - Ren, Gaili AU - Chang, Huoju AU - Zhang, Donglin 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 13
SP  - 1177
EP  - 1182
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1177.1182
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1177.1182
KW  - E. coli
KW  -Virulence Island
KW  -LEE
KW  -HPI
KW  -serotype
AB  - The detections of the Locus of Enterocyte Effacement Pathogenicity 
  Island (LEE) and High Pathogenicity island (HPI) in 26 <I>E. coli</I> samples 
  from chickens were conducted by PCR. <I>Ler</I> and <I>eaeA </I>genes located 
  in the core of LEE Virulence Island while <I>irp2</I>, <I>fyuA</I> and <I>asn_RNA_intB</I> 
  genes in HPI Pathogenicity Island were all detected. At the same time, O serotype 
  identification of 26 <I>E. coli</I> samples were conducted. The results showed 
  that the positive rate of <I>Ler</I> gene in LEE Virulence Island was 3.85% 
  (1/26) while eaeA was 3.85% (1/26). The rate of <I>fyuA</I> gene in HPI pathogenicity 
  island was 42.3% (11/26) while <I>irp2</I> was 73% (19/26), <I>irp2+fyuA+</I> 
  was 42.3% there was no <I>asn_RNA_intB</I> gene. The results of serotype identifications 
  showed that 15 strains related to serotype O4, O91, O78, O107, O38, O111, O88, 
  O53, O24, O9 and O11 except 11 strains. Among 15 strains, O91, O78 and O38 were 
  dominant serotypes whose proportion were 20 (3/15), 20 (3/15) and 26.7% (4/15), 
  respectively. The nucleotide sequence similarities were &gt;95% for 26 strains 
  isolated from chickens.
ER  -