TY  - JOUR
T1  - Establishment of a Murine αυβ1 Transgenic CHO-K1 Cell Line 
  and its Susceptibility to Foot-and-Mouth Disease Virus Type Asia l/HN/2006 in China
AU - Zheng, H.X. AU - Zhang, Y. AU - Zhang, Z.D. AU - Jin, Y. AU - Yang, F. AU - He, J.J. AU - Cao, W.J. AU - Sun, D.H. AU - Lv, L. 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 1
SP  - 108
EP  - 117
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.108.117
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.108.117
KW  - Foot-and-mouth disease
KW  -foot-and-mouth disease virus
KW  -integrin
KW  -receptor
KW  -cellular
AB  - Field isolates of Foot-and-Mouth Disease Virus (FMDV) were 
  found to use four αυ integrins (αυβ1, αυβ3, 
  αυβ6 and αυβ8) as cellular receptors. Researchers 
  established a stable Chinese Hamster Ovary clone K1 (CHO-K1) cell line expressing 
  the murine αυβ1 heterodimer (designated as CHO-K1-αυβ1) 
  using a highly efficient lentiviral-based gene transfer technology to deliver 
  murine αυ, Internal Ribosome Entry Site (IRES) and β1 genes into 
  cell chromosomes and the inserted genes were then transcribed from a Cytomegalovirus 
  (CMV) promoter. αυβ1 expression was stringently regulated by 
  Doxycycline (Dox) and was found to be stable. CHO-K1-αυβ1 cells 
  were susceptible to FMDV type Asia l/HN/2006. The plaque assay revealed that 
  the virus produced bigger and more plaques in CHO-K1-αυβ1 cells 
  (1.05x10<SUP>4</SUP> PFU mL<SUP>-1</SUP>) than in CHO-K1 cells. When sodium 
  heparin (1 and 2 mg mL<SUP>-1</SUP>) was used as the inhibitor, the number of 
  plaques in CHO-K1 cells were significantly decreased (4.0x10<SUP>3</SUP>-35 
  and 20 PFU mL<SUP>-1</SUP>), supported by time-course of replication and proliferation. 
  The number and size of plaques on CHO-K1-&#945;&#965;&#946;1 cells showed no 
  obvious change, indicating that the &#945;&#965;&#946;1 heterodimer expressed 
  on CHO-K1-&#945;&#965;&#946;1 can be used as an FMDV receptor.
ER  -