TY  - JOUR
T1  - Molecular Cloning, Tissue Distribution and Expression of the Porcine Cationic Amino Acid Transporter CAT3
AU - Zuo, Jianjun AU - Xia, Weguang AU - Xu, Mei AU - Dai, Fawen AU - Huang, Chao AU - Ye, Hui AU - Cao, Qingyun AU - Feng, Dingyuan 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 11
SP  - 1070
EP  - 1077
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1070.1077
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1070.1077
KW  - Cationic amino acid transporter
KW  -CAT3
KW  -SLC7A3
KW  -ontogenetic regulation
KW  -pigs
AB  - The Cationic Amino acid Transporter CAT3 (<I>HGMW</I>-approved 
  gene symbol SLC7A3; solute carrier family 7, member 3) plays a crucial role 
  in amino acid nutrition. In this study, researchers cloned and sequenced porcine 
  CAT3 and examined its expression in the porcine small intestine. A 2276 bp porcine 
  CAT3 cDNA fragment was obtained that includes a 127 bp 5'UTR, 1860 bp Open Reading 
  Frame (ORF) and 289 bp 3'UTR. The predicted porcine CAT3 has 619 amino acids 
  with a molecular weight of 66.88 kDa and is 92.4, 94.5, 89.7 and 89.8% identical 
  in amino acid sequence to human, cattle, mouse and rat CAT3, respectively. Real-time 
  RT-PCR analysis showed that porcine CAT3 transcripts are expressed across a 
  panel of tissues of Landrace pigs at the age of day 7 with the highest expression 
  in the brain and heart and moderate expression in the liver, kidney, muscle, 
  intestinal tract and lung. The CAT3 mRNA abundance was significantly higher 
  at day 1 than at other ages in the duodenum and colon (p&lt;0.05) however, it 
  was highest at day 30 in the jejunum (p&lt;0.05) and ileum. There was no significant 
  difference in the CAT3 mRNA abundance in either the duodenum or the jejunum 
  between Landrace and Lantang pigs (p&gt;0.05). The CAT3 mRNA abundance in the 
  ileum of Lantang pigs was significantly higher than that in the ileum of Landrace 
  pigs at day 30 (p&lt;0.05) however, the colon of Landrace pigs demonstrated 
  significantly higher CAT3 mRNA than that of Lantang pigs at day 1 (p&lt;0.05).
ER  -