TY  - JOUR
T1  - Expression and Study on Immunogenicity of <I>P12A-3A</I> Genes of Type O 
  Foot and Mouth Disease Virus in Recombinant Attenuated Goatpox Virus Strain
AU - Wang, Wenhui AU - Wang, Jianke AU - Zhang, Qiang AU - Wu, Lei 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 11
SP  - 1059
EP  - 1065
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1059.1065
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1059.1065
KW  - Promotor P7.5
KW  -P12A-3C gene
KW  -live vector vaccine
KW  -FMDV
KW  -immunogenicity
AB  - The <I>P12A</I> gene fragments and <I>3C</I> gene fragments 
  of O/China 99 strains of foot and mouth virus were ligated into bilateral tandem 
  Vaccinia virus promoter P7.5 which could initiate expression of <I>EGFP</I> 
  gene and the expression box p-EGFP-N1-P7.5-P12A-3C was constructed. The expression 
  box was ligated to the linear vector pUC119-TK after KpnI enzyme digestion by 
  blunt end ligation to obtain the recombinant plasmid pUC119-TK-EGFP-P7.5-P12A-3C. 
  The homologous recombination took place in the BHK-21 cell between the recombinant 
  plasmid and the Capripox virus attenuated strain by deleted <I>TK</I> gene. 
  The PCR identification, the Western blot analysis and immunological experiment 
  on mice were carried out. The results showed that the recombinant strain could 
  be subcultured in BHK-21 cell for 10 passages, expressing <I>P12A-3C </I>gene 
  which was 2900 bp by sequence analysis, the Western blot analysis demonstrated 
  that the expressed protein of recombinant plasmid pUC119-TK-EGFP-N1-P7.5-P12A-3C 
  in BHK-21 cells infected GTPV AV41 could be specifically recognized by the hyperimmune 
  serum to type O FMDV. Immunity assay showed that the expression protein had 
  immunogenicity in mice. All the results showed that the Goat Pox virus attenuated 
  strain which could express <I>P12A-3C</I> gene of type O FMDV was obtained.
ER  -