TY  - JOUR
T1  - Effects of Exogenous Fibrolytic Enzyme on <I>in vitro</I> Ruminal Fermentation 
  and Microbial Populations of Substrates with Different Forage to Concentrate Ratios
AU - Yao, Jun-Hu AU - Li, Chao-Yun AU - Cao, Yang-Chun AU - Li, Shi-Zhao AU - Xu, Ming AU - Liu, Chan-Juan AU - Yu, Zhi-Peng AU - Zhao, Xiang-Hui 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 10
SP  - 1000
EP  - 1006
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1000.1006
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1000.1006
KW  - Exogenous fibrolytic enzyme
KW  -ruminal fermentation
KW  -forage to concentrate
KW  -in vitro
KW  -microbial populations
AB  - This research was conducted to investigate the effects of 
  exogenous fibrolytic enzyme on <I>in vitro</I> ruminal fermentation and microbial 
  populations with substrates in different Forage to Concentrate ratios (F:C). 
  Four levels (0, 40, 80 and 120 U g<SUP>-1</SUP> dry matter substrate) of fibrolytic 
  enzyme were supplemented to buffered rumen fluid and incubated with three different 
  F:C ratios (80:20, 50:50 and 20:80) substrates at 39&deg;C. After 24 h incubation, 
  the results showed that in three different F:C ratios, fibrolytic enzyme significantly 
  increased (p&lt;0.001) total gas production, <I>in vitro</I> dry matter disappearance, 
  concentration of total volatile fatty acid and molar proportion of propionate 
  and decreased (p&lt;0.001) pH but it did not significantly (p&gt;0.05) affect 
  molar proportion of butyrate. And in the high (F:C 80:20) and medium (F:C 50:50) 
  forage substrates, fibrolytic enzyme increased (p&lt;0.05) the molar proportion 
  of propionate and decreased (p&lt;0.05) the ratio of acetate to propionate but 
  the effects did not observed in high concentrate (F:C 20:80) substrates. It 
  was also found that fibrolytic enzyme increased (p&lt;0.05) the number of <I>Fibrobacter 
  succinogenes</I> and methanogens in the F:C 80:20 substrate but there was no 
  significant (p&gt;0.05) effects on microbial populations in the F:C 50:50 and 
  20:80 substrates. The results indicated that the fibrolytic enzyme improved 
  the degradation of substrates and had different effects on the ruminal fermentation 
  pattern and microbial populations under three F:C ratio substrates. The efficiency 
  of fibrolytic enzyme on high and medium forage substrates was greater than low 
  forage substrates.
ER  -