TY  - JOUR
T1  - Cloning and Sequence Analysis of Donkey Growth Hormone Gene
AU - Zhu, Wenjin AU - , Yongmei Su AU - , Jianhua Wu 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 6
SP  - 731
EP  - 734
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.731.734
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.731.734
KW  - Donkey
KW  -growth hormone gene
KW  -cloning
KW  -sequence analysis
KW  -homology
KW  -China
AB  - The PCR primers were designed online according to result of gene homology comparison. Donkey <I>GH </I>gene DNA and cDNA sequence were cloned from liver and blood by RT-PCR and PCR, compared with <I>GH</I> gene sequences of different species by bioinformatics. The donkey <I>GH </I>gene sequence was 1928 bp including the 706 bp cDNA sequence with the complete CDS. By comparison for DNA and cDNA sequences, it was found that the <I>GH</I> gene sequence included 5 exons and 4 introns, encoding 216aa including signal protein of 26aa and matured protein of 190aa. Based on the analysis of the similarity of <I>GH</I> genes in different species on the level of cDNA, DNA and the deduced amino acid there was the most homology to the horse. The <I>GH</I> gene of donkey was conservative in the process of evolution and its promotor was not specific TATA box but was CATA box. The mutation of C&rarr;G in 1267 may affect the growth and development of donkeys and horses. All researches made an essential foundation for GH regulation of gene expression, evolution, polymorphism analysis in the future.
ER  -