TY  - JOUR
T1  - Relationship Between Fluoroquinolone Resistance and <I>gyrA</I>, <I>parC</I> Gene in <I>Streptococcus suis</I> Type 2 Isolates in Hebei Province China
AU - , Zengjun Ma AU - Rui, Ping AU - , QiuYue Wang AU - , Hai Fang AU - , JianHua Wu AU - , ZhiXin Fu AU - , Qing Hui Jia AU - , Xuexia Wen 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 6
SP  - 719
EP  - 722
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.719.722
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.719.722
KW  - Streptococcus suis
KW  -fluoroquinolone
KW  -parC gene
KW  -gyrA gene
KW  -amino acid
KW  -China
AB  - To detect the relationship between fluoroquinolone resistance and <I>gyrA</I>, <I>parC</I> gene mutation in <I>Streptococcus suis</I> type 2. The MIC of 14 strains of <I>Streptococcus suis</I> type 2 were determined by microdilution. The genes encoding the Quinolone-Resistance Determining Region (QRDRs) of parC and gyrA in fluoroquinolone-susceptible and resistant <I>Streptococcus suis</I> clinical isolates were identified and sequenced. Ser to Phe and Arg to Leu mutation at position 79 and 87 of the <I>parC</I> gene was detected in fluoroquinolone resistance SS2 and Arg to Ser or Ser to Arg mutations at the position 66 or 81 of <I>gyrA</I> gene were detected in 2 highly resistance strains; no amino acid changes in gyrA or parC were detected for 7 fluoroquinolone-susceptible strains; the mutations in both genes were found in the strains with MIC of fluoroquinolone &gt;32 &#956;g mL<SUP>-1</SUP>. Mutations in <I>parC</I> gene may result in low level resistance against fluoroquinolone and mutations in both <I>gyrA</I> and <I>parC</I> genes result in high level resistance.
ER  -