TY  - JOUR
T1  - Busulfan Influence the Survival Rate of Chicken Embryos and the Morphology of Gonads
AU - Yanzhang, Gong AU - Yu, Yang AU - Ping, Gong AU - Yanping, Feng AU - Yongping, Yang AU - Shijun, Li AU - Xiuli, Peng 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 4
SP  - 561
EP  - 565
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.561.565
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.561.565
KW  - Busulfan
KW  -testes
KW  -ovaries
KW  -chicken embryos
KW  -toxicity
KW  -China
AB  - Previous studies established the toxicity of busulfan (1, 4-butanediol dimethanesulfonate) suspension in sesame oil or emulsion to Primordial Germ Cells (PGC) and present study examined the survival rate of chicken embryos treated with different dosage of busulfan emulsion (0, 120, 240 360 and 480 &#956;g) then observed the morphology of testes and ovaries at 8.5, 11.5 and 14.5 days. The results showed that low dosage treatment (<120 &#956;g) did not have significant influence (p>0.05) on the survival rate of embryos compared with blank control and medium dosage treatment (240 and 360 &#956;g) significantly reduced the survival rate (p<0.05), the high dosage treatment (480 &#956;g) further reduced the survival rate (p<0.05). The increasing dosage of busulfan has positive correlation with both the death rate of embryos and the abnormal of the morphology structure in testes and ovaries. About <120 &#956;g busulfan treatment is recommended to obtain recipient chick embryo in producing germline chimera.
ER  -