TY  - JOUR
T1  - Cloning, Sequencing and Analysis of Melatonin Receptor Subtype MT<SUB>1</SUB> in Hypothalamus-Pituitary-Gonadal Axis and Pineal of Female Bactrian Camel
AU - Xingxu, Zhao AU - Junjie, Hu AU - Yong, Zhang AU - Junying, Wang AU - Hairong, Zhang 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 4
SP  - 470
EP  - 474
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.470.474
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.470.474
KW  - Clone
KW  -sequence
KW  -MT1
KW  -hypothalamus-pituitary-gonadal axis
KW  -bactrian camel
KW  -China
AB  - It is well known that melatonin is a coordinating signal for mammalian reproduction. In order to confirm the presence of melatonin receptors in hypothalamus-pituitary-gonadal axis and pineal of female Bactrian camel, the researchers used a Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) procedure to examine receptor MT<SUB>1</SUB> expression. The length of <I>MT<SUB>1</SUB></I> gene was 452 bp. RT-PCR assaying revealed the presence of the mt1 (Mel1a) melatonin receptor subtype in reproduction axis and pineal which were obtained from the slaughterhouse in the Ningxia Autonomous Region, China. Sequence has been confirmed a high identity (above 85%) with melatonin receptor MT<SUB>1</SUB> of other mammal known in GenBank. Comparing with other tissue&#146;s sequences, one base substitution changed the 108th TTC codon (encoding Phenylalanine) to TAC (Tyrosine) on hypothalamus. Although, there are base substitutions in pineal&#146;s gene, encoded amino acid are coincident with pituitary and ovary. The current results, the expression of MT<SUB>1</SUB> receptor mRNA in brain and ovary, suggest that melatonin regulate reproduction function through not only neuroendocrine but also directly acting on the ovary in Bactrian camel.
ER  -