TY  - JOUR
T1  - Haematological and Biochemical Alterations in Mice Following Experimental Infection with Whole Cell and Exotoxin (PLD) Extracted from <i>C. Pseudotuberculosis</i>
AU - Abdullah, Faez Firdaus Jesse AU - Abdullah, Rasedee AU - Sabri, Jasni AU - Haron, Abd Wahid AU - Saharee, Abdul Aziz AU - Osman, Abdinasir Yusuf 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 24
SP  - 4660
EP  - 4667
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4660.4667
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4660.4667
KW  - intraperitoneal exposure
KW  -exotoxin (PLD)
KW  -C. pseudotuberculosis
KW  -mice
KW  -thrombocytes
AB  - <I>Corynebacterium pseudotuberculosis</I> is the etiological 
  agent of caseous lymphadenitis, a chronic infectious disease of sheep and goats. 
  Despite the growing interest of this organism, little is known about the causality 
  and effect of this organism on haematological and biochemical values. The purpose 
  of this research, therefore was to determine haematological and serum biochemical 
  features in mice inoculated with whole bacterium and exotoxin (PLD) extracted 
  from <I>C. pseudotuberculosis</I>. The study was performed on apparently healthy 
  mice of 2-3 weeks old (n = 64). The mice were divided equally into 3 groups; 
  namely whole bacterium, exotoxin (PLD) and control group. Mice of whole bacterium 
  group were exposed intraperitoneally to 1 mL of the inoculums containing 10<SUP>9</SUP> 
  Colony-Forming Unit (CFU)/mL of live <I>C. pseudotuberculosis</I>. Exotoxin 
  group were exposed intraperitoneally with a single dose of exotoxin (PLD) extracted 
  from <I>C. pseudotuberculosis</I>. Mice served as a control group were challenged 
  intraperitoneally to 1 mL of Phosphate-Buffered Saline (PBS), pH 7. Following 
  post-infection, the mice were subjected for blood sample collection using cardiac 
  puncture method for haematological and biochemical analysis. The results of 
  this study revealed that there were significant decrease (p&lt;0.05) in Hb, 
  thrombocytes count and significant increase in WBC, neutrophils, monocytes counts 
  in the infected group. Biochemically, there were highly significant increase 
  (p&lt;0.05) in the mean levels of CK, AST, ALP and ALT. The mean level of albumin 
  in exotoxin group was significantly lower (p&lt;0.05) compared to <I>C. pseudotuberculosis</I> 
  and control groups while globulin level was significantly higher in <I>C. pseuodotuberculosis</I> 
  group after 48 h. In serum electrolytes, mean level of potassium and phosphate 
  were significantly (p&lt;0.05) higher in infected groups compared to control 
  group while there was no significant (p&lt;0.05) difference in the levels of 
  sodium, calcium and chloride. In conclusions, the present study of experimental 
  nature showed that <I>C. pseudotuberculosis</I> and its exotoxin (PLD) cause 
  disturbances in blood factors and electrolytes and pointed out that t hese haematological 
  and biochemical alterations should be taken into account in the context of diagnosis 
  and treatment of valuable infected hosts in order to prevent further consequences.
ER  -