TY  - JOUR
T1  - Cytokine and Chemokine Microarray Profiles in Lung and Hilar Nodes from Pigs after Experimental Infection with Actinobacillus Pleuropneumoniae
AU - Cui, Hengmin AU - Zuo, Zhicai AU - Peng, Xi AU - Deng, Junliang AU - Fang, Jing AU - Wang, Ya AU - Ren, Zhihua 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 24
SP  - 4603
EP  - 4610
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4603.4610
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4603.4610
KW  - Cytokine
KW  -chemokine
KW  -Actinobacillus pleuropneumoniae
KW  -microarray
KW  -China
AB  - The objective of this study was to determine cytokine and 
  chemokine microarray profiles in lung and Hilar Nodes (HN) from pigs infected 
  with Actinobacillus Pleuropneumoniae (APP). Twenty pigs were randomly assigned 
  to one of two groups: Control Group (CG) and inoculated with APP (TG). The infected-APP 
  pigs’ lung exhibited significantly (p<0.05) greater levels of chemokines 
  CCL2, CCL20, IL8 and slightly increase levels of chemokines CCL4, CCL5 and CXCL2 
  while significantly(p<0.05) decrease levels of chemokines CXCL10 and CXCL12. 
  APP infection significantly (p<0.05 or 0.01) stimulated expression of cytokines 
  IL-18, IL-6, TNF, GM-CSF, CASP3, CASP8 and significantly (p< 0.05 or 0.01) 
  suppressed expression of cytokines CD40, IRF1 in lung. Cytokines in infected-APP 
  pigs’ lung, IL-1A, IL-27, IRF3, IL-10 were slightly increased and CASP1, 
  IRF7, IL-12B, IL-2 were slightly decreased. Relative cytokine and chemokine 
  microarray data in HN indicated that APP infection significantly (p<0.05) 
  stimulated expression of cytokine IL-6 and significantly (p<0.05 or 0.01) 
  suppressed expression of cytokines CXCL12, CD40 and CASP1. In conclusion, 26 
  cytokine and chemokines mRNA expression levels in lung and HN obtained from 
  infected-APP or control swines were elucidated in this study. This research 
  provided evidence that the increased severity of lesions in the infected-APP 
  swines was associated mainly with alterations of cytokine and chemokines microarray 
  profiles, especialy in lung. The changes of all the cytokines in lung and HN 
  can lead stem cells to produce granulocytes (neutrophils, eosinophils and basophils) 
  and monocytes and also promoted neutrophil and macrophages to phagocytose bacterial 
  and foreign antigen at the site of inflammation. Defense function of pig infection 
  with APP was enhanced while immune function was weakened.
ER  -