TY  - JOUR
T1  - Construction and Transfection of EGFP-N1-BDNF Expression Plasmid
AU - Shen, Minren AU - Wang, Jianlong AU - Zhou, Xiaozhe AU - Xing, Xiaowei AU - Chen, Yanliang AU - Guo, Gangwen AU - Sun, Jianfeng AU - Zhang, Qingshan 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 24
SP  - 4599
EP  - 4602
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4599.4602
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4599.4602
KW  - Brain-derived neurotrophic factor
KW  -Alzheimer`s disease
KW  -obesity
KW  -lipofection
KW  -China
AB  - Brain-Derived Neurotrophic Factor (BDNF) is a member of the 
  neurotrophin family of growth factors and plays significant role in central 
  nervous system and the peripheral nervous system for supporting the survival 
  of existing neurons and encouraging the growth and differentiation of new neurons 
  and synapses. Increasing study indicate that BDNF is associated with neurological 
  disorders and psychiatric diseases. Beyond the importance of BDNF in nervous 
  system, compelling evidence also demonstrate that it is also essential for body 
  weight control and energy homeostasis. BDNF therefore, become the target in 
  various diseases research such as Alzheimer’s disease, depressive disorder 
  and obesity. In this study, researchers cloned the <I>BDNF</I> gene to p-EGFP 
  N1 expression plasmid, meanwhile, researchers also transfected the p-EGFP N1-BDNF 
  to Hela cell with lipofection. Fluorescence microscope and Western blot analysis 
  showed BDNF could normally express in Hela cell. However, the physiological 
  functions and pharmacological roles of the p-EGFP N1-BDNF need further validation 
  in cellular level and animal model.
ER  -