TY  - JOUR
T1  - Expression and Distribution Pattern of Ghrelin in the Gastrointestinal Tract of Saanen Dairy Goat
AU - Tong, Dewen AU - Yu, Gaoshui AU - Zhang, Wenlong AU - Huang, Yong AU - Ding, Li AU - Li, Wei AU - Li, Zhaocai AU - Xu, Dan AU - Dong, Feng AU - Du, Qian 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 24
SP  - 4585
EP  - 4592
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4585.4592
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4585.4592
KW  - Ghrelin
KW  -goat
KW  -gastrointestinal tract
KW  -immunohistochemistry
KW  -Western blot
AB  - Ghrelin, a gastric acylated peptide has been shown to express 
  in gastrointestinal tract of many animals. However, little is known about its 
  expression pattern in gastrointestinal tract of ruminants such as goat. In this 
  study, researchers investigated the expression and distribution of ghrelin in 
  the gastrointestinal tract of goat (Saanen dairy goat) including esophagus, 
  rumen, reticulum, omasum, abomasus, duodenum, jejunum, ileum, appendix, colon 
  and rectum by PCR, Immunohistochemistry (IHC) and Western blot. The PCR detection 
  showed that ghrelin mRNA existed in all the above tissues and was significantly 
  higher in the abomasus than in other tissues detected (p<0.05). Immunohistochemistry 
  assay showed that ghrelin-positive cells were observed in the mucosal layer 
  of all segments examined. The ghrelin-positive cells were most numerous in the 
  abomasus, less abundant in the duodenum, occasional in jejunum, ileum, appendix, 
  colon and rectum and rare in esophagus, rumen, reticulum, omasum and abomasus. 
  Western blot analysis confirmed the ghrelin protein expression pattern in the 
  gastrointestinal tract of the goat. These results demonstrate that ghrelin is 
  expressed in the gastrointestinal tract of Saanen dairy goat, especially in 
  the abomasus.
ER  -