TY  - JOUR
T1  - Somatic Cell Nuclear Transfer Efficiency Associated with Donor Cell Types in the Cashmere Goat
AU - Yuan, Jianglong AU - Jin, Yong AU - Zhu, Bing AU - Wang, Wei AU - Ren, Yu AU - Wu, Haiqing AU - Hao, Fei AU - Nuo, Mingtu AU - Jin, Muzi AU - Wang, Zhigang AU - Chang, Ming AU - Liu, Dongjun AU - Guo, Xudong 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 24
SP  - 4578
EP  - 4584
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4578.4584
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4578.4584
KW  - Cashmere goat
KW  -somatic cell nuclear transfer efficiency
KW  -cell type
KW  -embryo
KW  -China
AB  - The efficiency of donor cell types in cashmere goat Somatic 
  Cell Nuclear Transfer (SCNT) is undefined. In this study, SCNT was performed 
  using Caprine fetal Fibroblast Cells (CFCs), Caprine Ear Fibroblast Cells (CEFCs) 
  and Transgenic Caprine Fetal Fibroblast Cells (TCFCs) as donor cells to compare 
  the influences of cell type, transgene and sex of nuclear donor fibroblast cells 
  on the SCNT efficiency including fusion and cleavage rates of reconstructed 
  embryos and the birth, postnatal survival and mortality rates of cloned kids. 
  A total of 4,943 reconstructed embryos were obtained. Among them, 3,949 embryos 
  were fused and 3,737 embryos were cultured <I>in vitro</I> leading to a total 
  of 3,094 cleavage embryos. Furthermore, embryo transplantation analysis was 
  conducted on 1,873 cloned embryos with relatively normal morphology. A total 
  of 368 recipient goats were transplanted and 48 goats were born of which 35 
  goats survived.
ER  -