TY  - JOUR
T1  - Immunization Procedures of Δcrp Δasd Double Deleted Attenuated 
  Salmonella Enterica Serovar Typhimurium delivering Somatostatin Antigen in Mice
AU - Yang, Li-Guo AU - Liang, Ai-Xin AU - Dong, Fang-Xiao AU - Luo, Xuan AU - Yu, Xue AU - Han, Li AU - Han, Yan-Guo AU - Guo, Ai-Zhen 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 24
SP  - 4526
EP  - 4532
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4526.4532
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4526.4532
KW  - Somatostatin
KW  -Salmonella
KW  -dose
KW  -interval
KW  -mice
AB  - In order to estimate the optimal immunization procedures for 
  a novel somatostatin vaccine carried by Δcrp Δasd double deleted attenuated 
  Salmonella enterica serovar Typhimurium. Mice were vaccinated with somatostatin 
  vaccine strain (C500/pVGS/2SS-asd) by using three immunization dosages and three 
  alternative schedules. The specific antibody response was evaluated by indirect 
  ELISA Method. The results showed that 0.2x10<SUP>10</SUP> CFU per mouse was 
  the optimal immunization dosage to induce specific IgG response. Interestingly, 
  the booster groups either 2 weeks interval or 4 weeks interval after the first 
  immunization were not improving the level of specific IgG response as expected 
  compared with only injection one dose groups. Furthermore, the different interval 
  period of booster injections also affected the immune response, antibody levels 
  of booster given 4 weeks interval was much higher than that booster given 2 
  weeks interval. Similar results were obtained on body weight gains analysis. 
  The results of body weight gains of immunized mice also showed that the most 
  effective immunization dosage is 0.2x10<SUP>10</SUP> CFU per mouse and the optimal 
  immunization number is a single dose. These observations have a very important 
  application in the field of animal production.
ER  -