TY  - JOUR
T1  - The Effect of <i>Helicobacter pylori</i> on Apoptosis of the GES-1 Transfected pEGFP-N1-UreB Eukaryotic Expression Plasmid <i>in vitro</i>
AU - Chen, J.G. AU - Liang, A.X. AU - Han, L. AU - Xiong, J.J. AU - Guo, A.Z. AU - Yang, L.G. 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 23
SP  - 4435
EP  - 4440
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4435.4440
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4435.4440
KW  - pEGFP-N1-UreB
KW  -H. pylori SS1
KW  -GES-1
KW  -apoptosis
KW  -China
AB  - To investigate the effect of <I> Helicobacter pylori</I> on 
  apoptosis of the GES-1 transfected pEGFP-N1-UreB eukaryotic expression plasmid 
  <I>in vitro</I>. The <I>Hp-UreB</I> gene was amplified by PCR and cloned into 
  eukaryotic expression plasmid pEGFP-NI to construct recombinant plasmid pEGFP-NI-UreB 
  and transfected into GES-1 cells and screened by G418. GES-1 cells transfected 
  into plasmid pEGFP-NI-UreB were co-cultured with <I>H. pylori</I> SS1 and its 
  apoptosis was quantified by flow cytometry using Annexin V-APC and 7-AAD. Changes 
  in cell morphology were observed by transmission electron microscope. The recombinant 
  pEGFP-N1-UreB was constructed and stable transfected into GES-1 cells. The apoptotic 
  rates of cells transfected into pEGFP-N1-UreB plasmids were significantly lower 
  than those of control cells (p&lt;0.05).
ER  -