TY  - JOUR
T1  - Interferon-Gamma Enhance Immune Response and Efficacy of Recombinant Subunit Vaccine Against <i>Eimeria tenella</i> in Chickens
AU - Tao, Jianping AU - Xu, Jinjun AU - Peng, Jinbiao 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 23
SP  - 4394
EP  - 4400
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4394.4400
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4394.4400
KW  - Eimeria tenella
KW  -SO7 antigen
KW  -subunit vaccine
KW  -adjuvant
KW  -interferon-gamma
AB  - To effectively control coccidiosis with a subunit vaccine, 
  the refractile body antigen SO7 adjuvanted with Chicken Interferon-gamma (ChIFN-gamma) 
  was administered to chickens and its ability to protect against virulent challenge 
  by<I> E. tenella</I> was evaluated. About 5 days old chickens were subcutaneously 
  immunized with SO7 antigen (100 &#956;g per chick) mixed with various doses 
  of ChIFN-gamma or without adjuvant. A boost of the same dose was given at 12 
  and 19 days of age. The specific antibody response and lymphocyte proliferation 
  response to SO7 antigen were measured at 5, 12, 19 and 26 days of age. Chickens 
  in all groups were orally challenged with 1x10<SUP>5</SUP> homologous sporulated 
  oocysts of <I>E. tenella</I> at 26 days of age and killed at 8 days postchallenge. 
  Results showed that when immunized by antigen adjuvanted with ChIFN-gamma, specific 
  lymphocyte proliferation in chichens was obviously observed at 19 and 26 days 
  of age and specific antibody response was obviously observed at 12, 19 and 26 
  days of age. All groups administered with antigen mixed in adjuvant showed higher 
  protective immunity compared to PBS control, non-adjuvanted antigen control 
  in which the group given adjuvanted antigen with 5,000 units ChIFN-gamma showed 
  significantly stronger adjuvant effects in terms of lower mortality, oocyst 
  output, lesion score and higher weight gain (p&lt;0.05). Protective immunity 
  was affected by dose of the adjuvant, no linear dose relationship was observed. 
  These results suggest that ChIFN-gamma has a strong adjuvant effect at a appropriate 
  dose and may potentially serve as a vaccine adjuvant against avian coccidiosis.
ER  -