TY  - JOUR
T1  - Establishment and Biological Characteristics of Qing Kedan Chicken Embryonic Fibroblast Line for Genetic Resource Conservation
AU - Ma, Yuehui AU - Guan, Weijun AU - Zhang, Wenxiu AU - Li, Changli AU - Li, Xiangchen AU - Wang, Hui 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 23
SP  - 4374
EP  - 4381
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4374.4381
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4374.4381
KW  - genetic resource conservation
KW  -embryonic fibroblast line
KW  -Qing Kedan chicken
KW  -somatic cell cryopreservation
KW  -China
AB  - A fibroblast cell line of Qing Kedan chicken was successfully 
  isolated, purified and cryopreseved through direct culturing of explants, serial 
  passage and cryogenic techniques. Assays for biological characteristics were 
  conducted and the results suggested that: the cells cultured with a Population 
  Doubling Time (PDT) of about 20 h, constituted a typical fibroblast cell line 
  in morphology; tests for microbial contamination regarding bacteria, fungi, 
  viruses and mycoplasmas were exclusively negative; Lactate Dehydrogenase (LDH) 
  and Malate Dehydrogenase (MDH) polymorphism analysis disproved the existence 
  of cross-contamination from other cell lines; diploid cells making up 85-93% 
  of the population verified the hereditary stability of the fibroblast line. 
  pEGFP-N3, pEYFP-N1 and pDsRed1-N1 were transfected into the Qing Kedan chicken 
  embryonic fibroblasts with transfection efficiencies between 12.6 and 39.7%. 
  To locate their cellular distribution, transfected cells were observed at 24, 
  48 and 72 h with confocal microscopy and the fluorescences could be observed 
  throughout the cytoplasm and nuclei of positive cells except in cryptomere vesicles. 
  This research preserved the precious germplasm resource of Qing Kedan chicken 
  at cell level and hence served as an enlightening reference for those of other 
  poultry in the world.
ER  -