TY  - JOUR
T1  - Cloning and Expression of Chicken AvBD8 Mature Peptide Gene in <i>Lactococcus lactis</i>
AU - Xue, Xiuheng AU - Tu, Jian AU - Peng, Kaisong AU - Miao, Liu AU - Qi, Kezong 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 23
SP  - 4369
EP  - 4373
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4369.4373
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4369.4373
KW  - expression
KW  -cloning
KW  -AvBD8 mature peptide
KW  -Lactococcus lactis
KW  -China
AB  - Chicken Beta Defensin-8 (AvBD8) is an antibacterial peptide 
  that plays significant roles in innate immunity. The aim of this study was to 
  investigate the expression of Chicken avian AvBD8 mature peptide gene in <I>Lactococcus 
  lactis</I> (<I>L. lactis</I>) system. In this study, the <I>AvBD8</I> gene was 
  cloned and sequenced from chicken liver by Reverse Transcriptase-Polymerase 
  Chain Reaction (RT-PCR). Then, the AvBD8 mature peptide gene was amplified and 
  cloned into the expression vector pNZ8048. The result showed that the recombinant 
  vector pNZ8048-AvBD8 that verified by colony PCR, double digestion and DNA sequencing 
  was transformed into the competent cell <I>L. lactis</I> NZ9000 by electroporation. 
  The expression of the recombinant protein AvBD8 was confirmed by tricine SDS-PAGE 
  analysis. The present findings suggest that <I>L. lactis</I> could act as a 
  host for the production of avian beta defensins for further study.
ER  -