TY  - JOUR
T1  - Sequence Variation in <I>ROP9</I> Gene among <I>Toxoplasma gondii</I> Strains 
  from Different Hosts and Geographical Locations
AU - Zhou, Dong-Hui AU - Chen, Jia AU - Fang, Su-Fang AU - Li, Zhong-Yuan AU - Liu, Guo-Hua AU - Zhu, Xing-Quan 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 22
SP  - 4288
EP  - 4291
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4288.4291
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4288.4291
KW  - Toxoplasma gondii
KW  -toxoplasmosis
KW  -Rhoptry Protein 9 (ROP9)
KW  -sequence variation
KW  -phylogenetic analysis
AB  - <I>Toxoplasma gondii</I> infects a wide range of animals and 
  humans causing toxoplasmosis. <I>T. gondii</I> rhoptry protein 9 (TgROP9) is 
  expressed only in tachyzoite stage that might be involved in the early stages 
  of invasion and this soluble protein is constituted with putative B-cell epitope 
  but also can trigger a T cell response and exclusively react with CD4 + T cell 
  clone. In this study, researchers examined sequence variation in <I>ROP9</I> 
  gene among seven <I>T. gondii</I> strains from different hosts and geographical 
  localities by PCR amplification, sequence analysis and phylogenetic reconstruction 
  using Maximum Parsimony (MP). The results showed that the entire genome sequence 
  of the <I>TgROP9</I> gene was 4555 bp in length and no size variation was detected 
  among all the strains. In addition, there were 26 (0-0.7%) variable nucleotide 
  positions among all isolates with 9 variations of these being in the coding 
  region and the other 11 were distributed among the 4 introns. Phylogenetic analyses 
  revealed that the TgROP9 sequences were not a suitable genetic marker to differentiate 
  <I>T. gondii</I> strains of different genotypes from different hosts and geographical 
  locations. This study determined the TgROP9 genomic sequences of seven <I>T. 
  gondii</I> strains and demonstrated the existence of low sequence variation 
  in <I>TgROP9</I> gene, suggesting that <I>T. gondii</I> ROP9 may represent a 
  good vaccine candidate against toxoplamosis.
ER  -