TY  - JOUR
T1  - A Rapid Colorimetric Loop-Mediated Isothermal Amplification Assay for the Detection of <I>Lawsonia intracellularis </I>in Pigs
AU - Cho, Ho-Seong AU - Choi, Nag-Jin AU - Park, Byoung-Yong AU - Park, Chul AU - Kim, Young-Jun 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 22
SP  - 4258
EP  - 4262
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4258.4262
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4258.4262
KW  - Lawsonia intracellularis
KW  -loop-mediated isothermal amplification
KW  -LAMP
KW  -porcine proliferative enteropathy
KW  -pigs
AB  - A colorimetric Loop-mediated isothermal Amplification (LAMP) 
  assay with hydroxy naphthol blue was designed for the detection of <I>Lawsonia</I> 
  (L.)<I> intracellularis</I> which is an important pathogenic bacteria that causes 
  animal diseases. A set of six primers was designed to target the aspartate ammonia-lyase 
  (<I>asp</I>A) gene. Serial 10 fold dilutions of cultured <I>L. intracellularis</I> 
  and spiked feces were used for LAMP optimization. The lower limit of the linear 
  range of the assay in <I>L. intracellularis</I> was 10<SUP>1</SUP> <I>L. intracellularis</I> 
  which is ten times more sensitive than conventional PCR. Based on testing in 
  210 porcine fecal samples using LAMP and conventional PCR, the agreement quotients 
  between LAMP and conventional PCR was 0.92. These results suggest that colorimetric 
  LAMP is a simple method for <I>L</I>. <I>intracellularis </I>detection in porcine 
  fecal samples.
ER  -