TY  - JOUR
T1  - An Identification Method for the Tokyo X Strain; The Pig Brand of the Tokyo Metropolitan Agriculture and Forestry Research Center
AU - Okumura, Naohiko AU - Suzuki, Ayumi AU - Uehara, Yoshifumi AU - Uenishi, Hirohide AU - Awata, Takashi 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 22
SP  - 4236
EP  - 4245
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4236.4245
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4236.4245
KW  - Tokyo X strain
KW  -identification method
KW  -SNPs
KW  -exclusion probability
KW  -PCR-RFLP
AB  - In this study, researchers developed a DNA-based identification 
  method for the Tokyo X strain which is a pig brand developed by the Tokyo Metropolitan 
  Livestock Experiment Station. To formulate the Polymerase Chain Reaction-Restriction 
  Fragment Length Polymorphism (PCR-RFLP) Method, Single Nucleotide Polymorphisms 
  (SNPs) were screened and a total of 104 SNP loci were fixed in the original 
  Tokyo X strain of these, researchers selected 8 SNPs which are recognisable 
  by restriction endonucleases and have high polymorphism within other major commercial 
  breeds and market pork. The combined exclusion probability of general commercial 
  pork and Berkshire pork from Tokyo X was 98.1 and 97.4%, respectively. The SNP 
  marker set developed in this study will be useful in preventing fake Tokyo X 
  brand pork from appearing on the market.
ER  -