TY  - JOUR
T1  - Polymerase Chain Reaction for Diagnosis of Aujeszky Disease in Mexico
AU - Antonia Coba Ayala, Maria AU - Escatel, Guadalupe Socci AU - Zapata Salinas, Laura Elena AU - Carrera Salas, Maria Elvira AU - Castaneda, Enrique Chavez 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 22
SP  - 4217
EP  - 4220
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4217.4220
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4217.4220
KW  - Pigs
KW  -Aujeszky`s disease
KW  -pseudorabies virus
KW  -PCR diagnosis
KW  -DNA
KW  -optimal condition
AB  - The objective of this study was to establish the optimal conditions 
  for carrying out the PCR technique to detect the Aujeszky’s 
  Disease (AD) virus in tissues. For this purpose, researchers used different 
  rabbit and pig tissue inoculated with the Shope strain of the AD virus as well 
  as uninoculated animal tissues. Cell culture of the same strain was used as 
  positive control. A commercial kit was employed for DNA extraction. DNA amplification 
  was performed with a pair of primers flanking a 334 bp fragment of the <I>gB 
  (gII)</I> gene. It was possible to obtain the expected product in 14 of 16 samples 
  of tissues from infected animals. These results were consistent with those obtained 
  by virus isolation. The assay sensitivity showed that the PCR can amplify DNA 
  up to 65 fg. The specificity of the test was confirmed by failure to observe 
  any amplification product from the 16 samples neither from negative animals 
  nor from bovine herpesvirus DNA. It is a molecular method that may be useful 
  to complement the diagnosis of the disease in Mexico.
ER  -