TY  - JOUR
T1  - Evidence of a Heparin-Binding Protein in the Plasma of the Slipper Oyster, <I>Crassostrea iredalei</I>
AU - Najiah, M. AU - Zamani, A.I. AU - Nadirah, M. AU - Amiza, M.A. 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 22
SP  - 4147
EP  - 4155
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4147.4155
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4147.4155
KW  - Heparin-Binding Protein (HBP)
KW  -Crassostrea iredalei
KW  -2D-electrophoresis
KW  -proPhenoloxidase-activating (proPO) system
KW  -Malaysia
AB  - Recognition proteins play an important role in the immunodefense 
  system of invertebrates. The haemolymph of the Slipper oyster, <I>Crassostrea 
  iredalei</I>, contains a heparin-binding protein with a molecular weight of 
  35 kDa as determined by SDS-PAGE analysis. The protein was purified using column 
  chromatography. This binding protein possesses a serine protease activity thus 
  it is capable of activating the prophenoloxidase-activating (proPO) system. 
  However, the protein lacks &#946;-1,3-glucanase activity. Using rabbit antiserum 
  against the isolated protein in immunodiffusion and immunoblotting assays it 
  produced a single precipitant and a single band, respectively. However, N-terminal 
  amino acid sequence of BGBP: Threonine-Alanine-Arginine-Asparagine-Glutamic 
  acid-Alanine-Asparagine-Valine was similar to Cavortin (AAT44352) and extracellular 
  superoxide dismutase (AAY60161) from <I>C. gigas</I>.
ER  -