TY  - JOUR
T1  - The Regulatory Role of Colorectal Adenocarcinoma Hypoxia-Inducible Factor-1α in the Expression of N-myc Down-Stream Regulative Gene 1
AU - Zhao, Duan-Yi AU - Chen, Ming-Kai AU - Jiang, Cong-Qing AU - Gassimou, Bangoura AU - Liu, Zhi-Su AU - Cao, Zhuo 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 22
SP  - 4121
EP  - 4126
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4121.4126
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4121.4126
KW  - Hypoxia-inducible factor-1a
KW  -N-myc down-stream regulative gene 1
KW  -small interferring RNA
KW  -colorectal adenocarcinoma
KW  -China
AB  - HIF-1α participates in the neovascularization of colorectal 
  adenocarcinoma tumor and the development of tumor via regulating VEGF expression. 
  NDRG1 was over expressed in the process of colorectal adenocarcinoma. Current 
  studies demonstrate that hypoxia is a NDRG1-inducible factor and HIF-1α 
  also has taken part in this process. The study was to investigate regulation 
  relationship between HIF-1α and NDRG1 in the LS174T cells and the two proteins 
  associated with human colorectal carcinoma Duke classification. Construct siRNA 
  expressive carrier pSilence-2.1-U6-siRNA targeting Hypoxia-Inducible Factor-1α 
  (HIF-1α), hypoxially cultured LS174T cells were transferred by liposome. 
  HIF-1α and NDRG1 mRNA and protein expression level was analyzed by RT-PCR 
  and Western blot. In addition, 62 cases of clinical tissue specimen of human 
  colorectal carcinoma were collection to detect HIF-1 and NDRG1 expression level 
  using ISH, IHC. HIF-1α gene expression was inhibited by siRNA at transcriptional 
  and protein level in the LS174T cells under hypoxia conditions for 24 h. Meanwhile, 
  NDRG1mRNA expression was also significantly suppressed. In colorectal adenocarcinoma 
  organization, HIF-1α and NDRG1 positive expression was both significantly 
  correlated with Dukes staging (p<0.05). Moreover, spearman correlation analysis 
  displayed HIF-1α was correlated with NDRG1 (p<0.05). The over expression 
  of HIF-1α and NDRG1 likely play pivotal roles in the development of colorectal 
  gland adenoma canceration and colorectal adenocarcinoma.
ER  -