TY  - JOUR
T1  - Clone and Bioinformatics Analysis of Chinese-Belgium Rabbit Metallothionein-1 (<I>MT<SUB>1</SUB></I>), <I>MT<SUB>2</SUB></I> and <I>MT<SUB>3</SUB></I> Genes CDS Region
AU - Songjia, Lai AU - Ming, Zhang AU - Kun, Wang AU - Shiyi, Chen AU - Jie, Wang 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 21
SP  - 4034
EP  - 4039
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4034.4039
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4034.4039
KW  - Rabbit
KW  -Metallothionein (MT)
KW  -clone
KW  -bioinformatics analysis
KW  -China
AB  - Metallothioneins (MTs) play important roles in mental ion metabolism, detoxication of heavy mental, clearance of Reactive Oxygen Species (ROS). In the studies, Coding Sequences (CDS) of <I>MT<SUB>1</SUB></I>, <I>MT<SUB>2</SUB></I> and <I>MT<SUB>3</SUB></I> genes from Chinese-Belgium rabbit were cloned and sequenced. The results showed: CDS length of MT<SUB>1</SUB>, MT<SUB>2</SUB> and MT<SUB>3</SUB> was 186, 186 and 201 bp, respectively, encoded 61, 61 and 66 amino acids, respectively. Amino acid sequence of MTs includes 7 typical Cys-Xaa-Cys motifs and 20 cysteines which form 10 disulfide-bonds. Similarity analysis showed that the similarity of MT<SUB>1</SUB> and MT<SUB>2</SUB> reached to 87.6%; the similarity of MT<SUB>2</SUB> and MT<SUB>3</SUB> was 78.0%; the similarity of MT<SUB>3</SUB> and MT<SUB>1</SUB> was 76.3%. Alignment and cluster analysis of <I>MTs</I> genes from different species suggest MTs are high conserved but different subtype of <I>MTs</I> genes have separated and formed in the early stages of the evolution of species and then they individually evolve.
ER  -